Aspirantskiy Vestnik PovolzhiyaAspirantskiy Vestnik Povolzhiya2072-23542410-3764Samara State Medical University11112310.55531/2072-2354.2023.23.1.31-35Research ArticleMorphological and anatomical study of <i>Crataegus submollis</i> Sarg. shootsVolkovaNadezhda A.<p>Postgraduate Student of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy</p>n.a.volkova@samsmu.ruhttps://orcid.org/0000-0002-7820-7074KurkinVladmir A.<p>PhD, Professor, Head of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy</p>v.a.kurkin@samsmu.ruhttps://orcid.org/0000-0002-7513-9352PravdivtsevaOlga E.<p>PhD, Associate Professor, Professor of the Department of Pharmacognosy with Botany and the Basics of Phytotherapy</p>o.e.pravdivtseva@samsmu.ruhttps://orcid.org/0000-0003-3318-3168AndreevArkadii A.<p>Student of the Institute of Pharmacy</p>andreevarkadii2@gmail.comhttps://orcid.org/0000-0002-5328-1710KurkinaAnna V.<p>PhD, Associate Professor, Head of the Department of Pharmaceutical Technology with a Course of Biotechnologies</p>a.v.kurkina@samsmu.ruhttps://orcid.org/0000-0002-5028-9186PervushkinSergei V.<p>PhD, Professor, Professor of the Department of Pharmaceutical Technology with a Course of Biotechnologies</p>s.v.pervushkin@samsmu.ruhttps://orcid.org/0000-0002-7000-271XAgapovAlbert I.<p>PhD, Professor, Professor of the Department of Medical Chemistry</p>a.i.agapov@samsmu.ruhttps://orcid.org/0000-0001-5487-8992Samara State Medical University2103202323131352909202203122022Copyright © 2023, Volkova N.A., Kurkin V.A., Pravdivtseva O.E., Andreev A.A., Kurkina A.V., Pervushkin S.V., Agapov A.I.2023<p><strong>Aim</strong> to conduct a morphological and anatomical study of dried shoots of soft hawthorn collected during the flowering period.</p>
<p><strong>Material and methods.</strong> The shoots of soft hawthorn were collected during the flowering period in the Botanical Garden of Samara University. The micro-preparations were immersed in an alcohol-water-glycerin mixture (1:1:1). To determine the lignified elements of stems and pedicels, a solution of 2% aniline sulfate in 50% ethanol was used. The samples of raw material were examined in daylight using a magnifying glass and a "Motic DM-39C-N9GO-A" binocular. The microscopic examination was performed in transmitted and reflected light using a digital microscope Carl Zeiss Primo Star 415500-0057-000 (Imaging Software for Microscopy ZEN soge V2.7).</p>
<p><strong>Results. </strong>The study of anatomical and morphological properties of hawthorn shoots revealed their most characteristic feature - the abundant pubescence with simple hairs. In addition, the edge of the leaf blades, bracts and sepals had multicellular glands with brown contents.</p>
<p><strong>Conclusion.</strong> The anatomy of the petiole of the hawthorn leaf was described for the first time. The study allows us to identify characteristic changes in the conducting system of the leaf. The morphological and anatomical analysis allows us to identify the common features of the semi-soft hawthorn and closely related species.</p>Crataegus submollis Sarg.flowersleavesstemsshootsmorphological and anatomical analysisCrataegus submollis Sarg.цветкилистьястеблипобегиморфолого-анатомический анализ[Russian Federation State Pharmacopeia. XIVth edition. M., 2018. (In Russ.). [Государственная фармакопея Российской Федерации. XIV издание. М., 2018]. Available et: http://femb.ru/femb/pharmacopea.php][Kurkin VA, Pravdivtseva OE, Shaikhutdinov IKh, et al. Species of the genus Crataegus L.: standardization and development of drugs. Samara, 2020. (In Russ.). [Куркин В.А., Правдивцева О.Е., Шайхутдинов И.Х., и др. Виды рода боярышник (Crataegus L.): стандартизация и создание лекарственных препаратов. Самара, 2020].][European Pharmacopoeia. 6th Ed. Rockville: United States Pharmacopoeial Convention, Inc., 2008.][Sagaradze VA, Babaeva EY, Kalenikova EI. The authentification criteria of the promising form of raw material – Crataegus flowers with leaves. Problems of biological, medical and pharmaceutical chemistry. 2017;2:26-31. (In Russ.). [Сагарадзе В.А., Бабаева Е.Ю., Каленикова Е.И. Установление подлинности перспективного вида лекарственного сырья – цветков с листьями боярышника. Вопросы биологической, медицинской и фармацевтической химии. 2017;2:26-31].][Trees and bushes of the USSR. Moscow–Leningrad, 1954. Vol. 3. (In Russ.). [Деревья и кустарники СССР. Москва–Ленинград, 1954. Т. 3].][Morozova TV, Kurkin VA, Pravdivceva OE, et al. Comparative phytochemical study of fruits, cormus and flowers of some species of the genus hawthorn. Aspirantskij vestnik Povolzh'ya. 2018;1-2:22-25. (In Russ.). [Морозова Т.В., Куркин В.А., Правдивцева О.Е., и др. Сравнительное фитохимическое исследование плодов, побегов и цветков некоторых видов рода боярышник. Аспирантский вестник Поволжья. 2018;1-2:22-25].][Morozova TV, Kurkin VA, Zajceva EN, et al. Antidepressant activity of Crataegus sanguinea Pall. extracts. Pharmacy. 2017;4:37-39. (In Russ.). [Морозова Т.В., Куркин В.А., Зайцева Е.Н., и др. Антидепрессантная активность экстрактов боярышника кроваво-красного. Фармация. 2017;4:37-39].][Sagaradze VA, Babaeva EYu, Ufimov RA, et al. Total flavonoids in Crataegus “flowers with leaves” raw material of Russian flora. Chemistry of plant raw material. 2018;4:95-104. (In Russ.). [Сагарадзе В.А., Бабаева Е.Ю., Уфимов Р.А., и др. Содержание флавоноидов в цветках с листьями боярышников (Crataegus L.) флоры РФ. Химия растительного сырья. 2018;4:95-104].][Khasanova SR, Krivoshchekov SV, Kudashkina NV, et al. Study on the polysaccharides of Crataegus sanguinea (Rosaceae) leaves from flora of Bashkortostan Republic. Rastitelnye resursy. 2015;3:397-406. (In Russ.). [Хасанова С.Р., Кривощеков С.В., Кудашкина Н.В., и др. Компонентный состав полисахаридного комплекса листьев Crataegus sanguinea (Rosaceae) из флоры Республики Башкортостан. Растительные ресурсы. 2015;3:397-406].]