Email this article The effect of the sulfated glycosaminoglycans complex on the human corneal epithelial cells proliferation: an in vitro experiment
- Authors: Trifanenkova I.G.1, Petersen E.V.2, Novikov S.V.3, Usanova G.Y.4
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Affiliations:
- S.N. Fedorov Eye Microsurgery Federal State Institution, Kaluga Branch
- Moscow Institute of Physics and Technology (National Research University)
- Scientific and experimental production Eye Microsurgery
- Eye Microsurgery Center
- Issue: Vol 22, No 2 (2022)
- Pages: 56-61
- Section: OPHTHALMOLOGY
- URL: https://aspvestnik.ru/2410-3764/article/view/108409
- DOI: https://doi.org/10.55531/2072-2354.2022.22.2.56-61
- ID: 108409
- Retraction date: 06.02.2023
- Retraction reasons description:
The official statement of the author about the discovery of a violation of publication ethics in terms of non-compliance of all the above members of the team of authors with the ICMJE authorship criteria: not all authors listed in the article made a significant contribution to the study and preparation of the article, approved the final version of the article and agreed to its publication.
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Abstract
Aim – to study the proliferative effect of various concentrations of sulfated glycosaminoglycans (sGAG) on the culture of human corneal epithelial cells.
Material and methods. The study focused on the properties of a mixture of sGAG consisting of chondroitin-4-sulfate, chondroitin-6-sulfates and keratan sulfates obtained by isolation from the transparent unchanged stroma of the cornea of farm animals. The material for the in vitro study was the cells of the anterior epithelium of the human cornea.
In the experimental group No.1, a mixture of sGAG was added to the culture medium at a concentration of 0.1%, in the experimental group No.2 – at a concentration of 0.5%, in the experimental group No.3 – at a concentration of 1.0%. In the control group No.1, the medium was replaced without the addition of solutions, cell seeding was carried out similarly to seeding with experimental samples. The negative control group included the holes of the tablet without the addition of medium and solution.
Results. The highest levels of the cellular index (CI) were obtained in the experimental group No.3 (1.00% of sGAG) and amounted to 2.86± 0.11 (the total increase in indicators was 60.67%). In the experimental group No.2 (0.50% sGAG), the cellular index levels were 2.65±0.24 (the total increase in indicators was 52.29%), which was significantly different from the experimental group No.1 (0.10% sGAG) and the control group No.1, where these indicators were 2.54± 0.21 (35.81%) and 2.37±0.02 (25.39%) respectively.
Conclusion. The final CI indicators show that solutions of the sGAG mixture at concentrations of 0.5% and 1.0% have a more pronounced proliferative effect on human corneal epithelial cells in vitro from the range of concentrations under study. The results obtained open up the prospects for the use of sGAG in the development of new approaches to the treatment of patients undergoing a chronic drug therapy or patients with concomitant corneal diseases.
About the authors
Irina G. Trifanenkova
S.N. Fedorov Eye Microsurgery Federal State Institution, Kaluga Branch
Author for correspondence.
Email: nauka@eye-kaluga.com
ORCID iD: 0000-0001-9202-5181
PhD, Deputy Director for Scientific Work
Russian Federation, KalugaElena V. Petersen
Moscow Institute of Physics and Technology (National Research University)
Email: petersen.elena.v@gmail.com
ORCID iD: 0000-0003-1924-3582
PhD, the Head of the Laboratory of Molecular Biological and Neurobiological Problems and Bioscreening of the Center for the Introduction of Genomic Technologies
Russian Federation, MoscowSergei V. Novikov
Scientific and experimental production Eye Microsurgery
Email: snovikov@ya.ru
Deputy General Director for Production
Russian Federation, MoscowGalina Yu. Usanova
Eye Microsurgery Center
Email: usanova-fit@mail.ru
ORCID iD: 0000-0001-8560-0651
Head of the Department of Laser Vision Correction, Ophthalmic Surgeon
Russian Federation, BryanskReferences
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